2001-03-28 07:42:47爛藍懶人
4th Try
Condition: 白天變長了
Temperature: 25 C
Aim: try 3 different concentrations of agarose gel. 1.1% 1.8% 2.5%, consider with xylene cyanol loading buffer
Discussion:
1. 1.1 %, ran over, only 1000 bp band showed on the gel.try again with other loading buffer.
2. 1.8 %, good seperation between 400bp~600bp. similar result as 2nd try (II)
3. 2.5 %, the bigger pieces of DNA didn't run very far, but the small pieces, not very good gel this time, need to try again.
Conclusion:
DNA run slower as increase concentration of agarose gel.
Temperature: 25 C
Aim: try 3 different concentrations of agarose gel. 1.1% 1.8% 2.5%, consider with xylene cyanol loading buffer
Discussion:
1. 1.1 %, ran over, only 1000 bp band showed on the gel.try again with other loading buffer.
2. 1.8 %, good seperation between 400bp~600bp. similar result as 2nd try (II)
3. 2.5 %, the bigger pieces of DNA didn't run very far, but the small pieces, not very good gel this time, need to try again.
Conclusion:
DNA run slower as increase concentration of agarose gel.