Condition: 呼~~信心大增,(既然有人看,那就更努力寫啦)
Temperature: room temp 25C
Equipmet : basic equipment for electropherosis.
Chemicals: ethidium bromide, 1xTBE buffer. marker, PCR sample, metaphor agarose gel powder.

Procedure:
follow the basic procedure.

Discussion:
1. making 1.8% Metaphor agarose gel (flask instead of beaker)
2. to prevent water loss during boiling, weight before and after
3. gel was run in 5V/cm this time, took more than one hour to run half way thru
4. the resolution, not good enough for 500 bp

Conclusion:
increase the power supply for two reasons,to run gel faster, and have better resolution.