楊惠郎 教授

職稱

電話

+886-6-275-7575 ext. 65601

傳真

+886-6-2766505

電子信箱

hlyang@mail.ncku.edu.tw

實驗室

水產動物疾病實驗室(台南市大學路一號成功校區資訊大樓六樓)

開授課程

97學年度第一學期

書報討論乙 (一)

專利實務特論

96學年度第二學期

書報討論乙 (二)

研究特論 (二)

生物技術專利特論

學歷

1. 加州大學(Davis) Bacteriology Ph.D(1972)
2. 國立台灣大學農化系學士(1966)

經歷

1. 國立成功大學特聘教授(2002- )
2. 國立成功大學生物科技研究所所長(2000- )
3. 國立成功大學生物科技研究所教授(2000- )
4. 國立成功大學生物科技中心副主任(2000- )
5. 國立成功大學農業生物技術研究中心主任(2000- )
6. 中央研究院生物農業科學研究所籌備處研究員(1998- )
7. 國立台灣大學獸醫系兼任教授(1994- )
8. 財團法人生物技術開發中心分子生物組主任)(1991-1997)
9. 國立陽明醫學院客座教授(1990-1991)
10. Managing Director of Research and Development, ENZO Diagnostics Inc.(1988-1990)
11. Director, Recombinant DNA / Microbiology, ENZO Biochem, Inc.(1984-1988)
12. Associate Director, Recombinant DNA, ENZO Biochem, Inc.(1982-1984)
13. Associate, Department of Genetics, The Public Health Research Institute in the City of New York (1980-1982)
14. Adjunct Assistant professor, New York University, School of Medicine (1980-1982)
15. Senior Research Associate, Department of Biological Sciences, Columbia University (1977-1980)

專長及研究領域

1. 生物技術
2. 疫苗技術
3. 檢驗試劑
4. 科技管理

榮譽及獎勵 (至少五年內)

1. l986  NIH  Technique Ad Hoc committee for SBIR grant
2. l987  NIH  Technique Ad Hoc Committee for SBIR grant
3. Invited lecture speaker in the l987 DNA Probe Conference held in San Diego,  CA.. sponsored by AACC
4. Invited lecture speaker in the l987 "Immunological Conference" held in Sarasota Fl. sponsored by Engineer Foundation
5. 行政院81年度傑出科技人才榮譽獎--醫學生物技術
6. Member of editorial board of  Asia-Pacific Journal of Molecular Biology and Biotechnology
7. Food Science and Agricultural Chemistry Journal: Editorial board 1999
8. 國科會生物處87年度甲種 研究獎勵獎 

專利 (至少五年內)

1. 中華民國專利: 發明第227114號：水生動物用口服疫苗 2005年2月1日至2022年9月4日
2. US 6,777,247, Nervous necrosis virus protein (Aug.17, 2004)
3. US Application No.: 09/946,273 , ORAL VACCINES Filing Date: September 5, 2001 , Approved date. Nov. 2004.
4. 0124124: Methods and materials useful for the analysis and detection of genetic material ( Inventor : Yang, Kirtikar and Pergolizzi ).Publication date: 1984. 07.11 Country designated:11: AT, BE, CH, DE, Fr, GB, IT, LI, LU, NL, SE
5. 0133473: In Vivo labeling of polynucleotide sequuences ( Inventor: Stra-        vrianopoulus, Yang and Kelker ). Publication date: 1985. 02.27 Country designated: 11: AT, BE, CH, DE, FR, GB, IT, LU, LI, NL, SE
6. 0294595: Polynucleotide composition and method for its production ( Inventor : Yang and Donegan ). Publication date: 1988. 12.14 Country designated: 13: AT, BE, CH, DE, ES, FR, GB, IT, LI, LU, NL, SE
7. 0237737: Nucleic acid probes specific for Neisseria gonorrhea and method for screening for a nucleic acid sequence specific for a genetically distinct group (Inventor: Lo and Yang ). 1987. 09. 23 Country designated: 9: BE, CH, DE, FR, GB, IT. LI, NL, SE
8. 0343424: Method for sample adherence to test substrate (Inventor: Yang, Todd and Jou ) . Publication date: 1989.11.29 Country designated: 4: DE, FR, GB, IT
9. 4900659: Nucleotide sequence composition and detection of Neisseria  gonorrheae and method for screening for a nucleotide sequence that is specific for a genetically distinct group (Inventor : Lo and Yang ) . 1990. 2. 13
10. 4987065: In vivo labeling of polynucleotide sequences (Inventor : Stravrianopoulus, Yang and Kelker ). 1991. 6. 22
11. 5024933: Method and kit for sample adherence to test substrate ( Inventor :     Yang, Todd nd Jou ). 1991. 6. 18
12. 1299073 : April 21, 1992 Nucleotide sequence composition and method for detection of Neisseria gonorrhoeae and method for screening for a nucleotide sequence that is specific for genetically distinct group Inventor: Andrew Lo and Huey-Lang Yang
13. 1338856: Jan 21, 1997 In vivo labeling of polynucleotide seqeunce Inventor: N. Kelker, J. Stavrianopoulos, Huey-Lang Yang
14. 1,339,249: Aug. 12,1997 Method for sample adherence to test substrate Inventor : H-L. Jou, J. Todd and Huey-Lang Yang Yang
15. 1,340,100:Oct 27, 1998 Polynucleotide composition and method Inventor : J. Donegan and Huey-Lang Yang
16. 2607058   Feb 13, 1997 A method for in vitro labeling polynucleotide Stravrianopoulus, Yang and Kelker
17. 2540482  July 8, 1996 In vitro labeling of polynucleotide sequence Stravrianopoulus, Yang and Kelker
18. 081902 1996.10.17製備台灣型EB病毒的複製促進因子(ZEBRA)抗原蛋白質之方法
19. 080691 1996.10.01利用基因工程技術製備具有EB病毒DNA polymerase 抗原蛋白質之方法
20. 83108294,371671本土性豬假性狂犬病毒gE之基因及其醣蛋白質，其製備方法及用途 (1999年)
21. 本土性豬假性狂犬病gX-gP63-gp50-gI基因及TK基因之核酸序列與gI基因及TK基問缺損病毒之製法及用途 (1995年申請,審核中)
22. 本土性豬假性狂犬病毒之二或多價變異株，及其製法與用途(1996年申請,審核中)
23. 使用去毒性蛋白研發免疫生育調控醫藥(1996年申請中)
24. Nucleotide sequences of Nervous Necrosis Virus and its application(申請美國及歐盟美專利中)
25. A fish oral vaccine method with bioencapsulation of recombinant bacterium(申請美國及歐名專利中)

著作 (至少五年內)

1. Review: 蕭振文，戴謙，楊惠郎。1997。以免疫去勢調控動物之生殖。科學農業。 45(5,6):176-185 .
2. Review: 蕭振文，劉瑞珍，戴謙，楊惠郎，宋永義。1997。逢機複製多態性核甘酸在畜產之應用。科學農業45(9,10):275-283.
3. 蕭振文，戴謙，宋永義，楊惠郎。1998。核酸疫苗研發之動物模式。科學農業 46:(5, 6): 209-216。
4. 蕭振文，施勇綸，楊惠郎，戴謙，宋永義。1998。生長分化因子之生物功能。科學農業46(11,12)：366-375。
5. 楊惠郎，蕭振文，戴謙，宋永義。1998。生物技術在畜產上之應用。畜產研究 31: (2) 197-210。
6. M.Y.Liu, Y.L.Chang, J.Ma, H.L.Yang, M.W.Hsu, C.J.Chen, J.Y.Chen, C.S.Yang。1997。 Evaluation of multiple antibodies to EB us as markers for detecting patients with nasopharyngeal carcinoma J. of Medical Virology 52:262-269 .
7. Review：Huey-Lang Yang。1999。The application of new biotechnology for disease prevention in animal pp. 73-82 in “ Biotechnology in agriculture in Asia, Asian productivity Organization.
8. J.W.Shiau, T.K. Tang, Y.L. Shih, C.Tai, Y.Y.Sung, J.L.Huang, H.L.Yang.。2000。 Mice immunized with DNA encodes a modified Pseudomonas aeruginosa exotoxin A develops protective immunity against exotoxin intoxication . Vaccine Vol. 19, issue 9-10, 1106-1112(SCI) .
9. J.P.R. Rajan, Carmen Lopez, John Han-You Lin, Huey-Lang Yang. 2001。Infection of Vibrio alginolyticus on cultured cobia, Rachycentron canadum in Taiwan. Bulletin of the European Association of Fish pathologist 21(6), 228-235(SCI)
10. Carmen Lopez, J.P.R. Rajan, John Han-You Lin, Tsun-Yung Kuo & Huey-Lang Yang. 2002 Disease outbreak in seafarmed Cobia (Rachycentron canadum) associate with Vibrio spp., Photbacterium damselae ssp piscida, monogenean and mycosporean parasites Bulletin of the European Association of Fish pathologist 22(3), 206-211.
11. Lopez C, J.P.R. Rajan, John Han-You Lin, Tsun-Yung Kuo & Huey-Lang Yang. (2002)Disease outbreak in seafarmed Cobia (Rachycentron canadum) associate with Vibrio spp., Photbacterium damselae ssp piscida, monogenean and mycosporean parasites Bulletin of the European Association of Fish pathologist 22 (3), 206-211.
12. P.R. Rajan, John H-Y Lin, M-S. Ho and H-L. Yang, (2003) Simple and rapid detection of Photobacterium damselae subsp. piscida by a PCR technique and plating method Jour of Applied Microbiology ,(J. of Applied Microbiology 2003, 95, 1375-1380. (PDF download)
13. H.S. Kuo, Y-L Su, H-L. Yang, and T.Y. Chen, (2005) Identification of Chinese medicinal fungus Cordyceps sinensis by PCR-single stranded conformation polymorphism and phylogenetic relationshipJ. Agri. Food Chem. (PDF download)
14. Yang, H.-L., Chien-Chih Yu, and John Han-You Lin, ?Fish oral delivery system using Artemia encapsulated with recombinant E. coli. International Association for Biologicals.: Progress in fish vaccinology. (ed) Midlyng PJ, Dev. Biol. Basel, Kreger, 2005, Vol. 121, pp 175-180, 2005
15. Han-Tso Lin, Han-You Lin, Huey-Lang Yang,( 2005) Studies of the Histology and Histochemical Enzyme-Staining Patterns of Major Immune Organs in Malabar Grouper (Epinephelus malabaricus, Bloch et Schneider), Journal of Fish Biology 66, 729-740. (PDF download)
16. Chen SP, Yang HL, Her GM, Lin HY, Jeng MF, Wu JL, Hong JR. (2006) Betanodavirus induces phosphatidylserine exposure and loss of mitochondrial membrane potential in secondary necrotic cells, both of which are blocked by bongkrekic acid. Virology 347(2):379-91. (PDF download)
17. Chen YM, Su YL, Lin JH, Yang HL, Chen TY. (2006)Cloning of an orange-spotted grouper (Epinephelus coioides) Mx cDNA and characterisation of its expression in response to nodavirus. Fish Shellfish Immunol 20(1):58-71. (PDF download)
18. Chen SP, Yang HL, Lin HY, Chen MC, Wu JL, Hong JR. (2006)Enhanced viability of a nervous necrosis virus-infected stable cell line over-expressing a fusion product of the zfBcl-xL and green fluorescent protein genes. J Fish Dis 29(6):347-54. (PDF download)
19. Kuo H-C, Su Y-L, Yang H-L., Huang I-C, and Chen T-Y*. Differentiation of Cordyceps sinensis by A PCR-Single-Stranded Conformation Polymorphism Based Method and Characterization of the Fermented Products in Taiwan. FOOD BIOTECHNOLOGY 2006;20:161-170.
20. John Han-You Lin, Tzong-Yueh Chen, Ming-Shyan Chen, Huey-En Chen, Ray-Liang, Chou, Z. Y. Cheng, M. S. Su, and Yang, H.-L.* Vaccination with three inactivated pathogens of cobia (Rachycentron canadum) stimulates protective immunity AQUACULTURE 255, 2006:125-132
21. Lin C.-C *, Lin J H-Y *, Chen M.-S and Yang, H.-L.*. An oral Nervous Necrosis Virus vaccine that induces protective immunity in larvae of grouper (Epinephelus coioides). AQUACULTURE 2007;268:265-273
22. Ou M-C, Chen Y-M, Jeng M-F, Chu C-J, Yang, H.-L., and Chen T-Y*. Identification of Critical Residues in Nervous Necrosis Virus B2 for dsRNA-binding and RNAi-inhibiting Activity through by Bioinformatic Analysis and Mutagenesis. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 2007;361:634-640
23. Chen Y-M, Wei C-Y, Chien C-H, Chang H-W, Huang S-I, Yang, H.-L., and Chen T-Y*. 2007. Myostatin Gene Organization and Nodavirus-influenced Expression in Orange-spotted Grouper (Epinephelus coioides). Comp. Biochem. Physiol. D, Genomics Proteomics 2:215-227
24. Lin H-T, Lin J H-Y, Lopez C, Chen Tzong-Yueh, Chen M-S, and Yang H-L. 2008. A Comparison of the Expression of immunity-related rag-1 and ikaros Genes with Histogenesis of the Thymus in Epinephelus malabaricus (Bloch & Schneider). Aquac. Res. 39:252-262. (PDF download)
25. Chen Y-M, Su Y-L, Shie P-S, Lin J H-Y, Yang H-L, and Chen Tzong-Yueh. 2008. Grouper Mx Confers Resistance to Nodavirus and Interacts with Coat Protein. Dev. Comp. Immunol. 32:825-836. (PDF download)
26. Kuo H-C, Su Y-L, Yang, H.-L., and Chen T-Y*. 2008. Establishment and Application of PCR-SSCP Profile for Molecular Typing of Cordyceps Fungi. Revised to Food Biotechnol. In press(SCI)

B. Conference papers

a.國際會議

1. Keynote speaker: Development of disease prevention system for warm water fish aquaculture, 2004
International Conference of Marine science and technology, 13-14, 2004, Kao-Hsiung, Taiwan

2. Invited speaker. The development of an oral Nervous Necrosis virus vaccine and its efficacy test in grouper, International Biotech Conference & Exhibition (IBC&E) Nov. 25-27, 2004, Ka0-Hsing, Taiwan

3. Invited oral presentation: Fish oral vaccine with recombinant E. coli. encapsulated in brine shrimp induces protective immunity 2003, 3rd International Symposium of fish vaccinology. Bergen, Norway.

4. Invited oral presentation: The development of fish disease system December 1-4, 2003
International marine biotechnological science & industry conference at National Museum of Marine Biology and Aquarium, Taiwan

5. Invited speaker: The development of vaccination program for NNV infection in grouper, 2002
International symposium on agricultural biotechnology.

6. Han-Tso Lin, C. Lopez, H. Y. Lin and H-L. Yang. How soon the grouper can arm itself?  International symposium on agricultural biotechnology, Dec. 13-14, 2002 Poster award

7. H.C. Kuo, H.L. Yang and T. Y. Chen, Phylogenetic analysis of the Chinese herb medicine Cordycepes sinensis based on 18 S rRNA gene.  International symposium on agricultural biotechnology, Dec. 13-14, 2002

6. Invited speaker: Immuno-response to killed and recombinant subunit vaccines of Nervous necrosis Virus in groper. 2001
Taiwan-Norway-USA International Symposium on fish virus pathogenesis and disease control, 2001. Sept. 27-31

7.Invited speaker: Fish oral vaccine method combining bio-encapsulation and recombinant DNA technology,2001
Asian aquaculture forum Nov. 25-29, 2001

8. Invited speaker: The application of biotechnology for the development of fish viral vaccine, Taiwan-Japan conference in Biotechnology for Aquatic animals, April 2001, Tokyo Japan

9.  Invited speaker: Fish oral vaccine method combining Bio-encapsulation and recombinant DNA technology. Taiwan-Norway-USA International Symposium on fish virus pathogenesis and disease control 2001. Sept. 27-31.

10 Invited speaker: Immuno-response to killed and recombinant subunit vaccines of Nervous necrosis Virus in groper. Taiwan-Norway-USA International Symposium on fish virus pathogenesis and disease control   2001. Sept. 27-31

11.H.L.Yang. (2001) The development of recombinant subunit vaccine for Nervous Necrosis Virus  International Conference on fish disease: European Fish Pathologist Dublin, Sept. 7-12, 2001.

12.Vibrosis in cultured cobia, Rachycentron canadum, L in Taiwan, Asian aquaculture forum  Nov. 25-29, 2001.

13.Invited speaker: Fish oral vaccine method combining bio-encapsulation and recombinant DNA technology, Asian aquaculture forum Nov. 25-29, 2001.

14.Immuno-response to killed vaccine and recombinant subunit vaccine of nervous necrosis Virus in grouper, Asian aquaculture forum Nov. 25-29, 2001.

15.Poster session: Cheng-Chieh Wang, John Han-You Lin, Chin-Chiu Lin, Tzong-Yueh Chen, and Yang H.-L. Cloning of rag-1 gene from cobia, Rachycentron canadum, and its expression during ontogenesis 2003, 3rd international Symposium of fish vaccinology

16.Poster session: John H.Y. Lin*, T. Y. Kuo+, H.C. Liang*, I.C. Huang#, and Yang H.-L.*,# The development and characterization of cell lines of grouper (Epinephelus coioides) and cobia (Rachycentron canadum) 2003, 3rd international Symposium of fish vaccinology

17.Lin S-C, Yang H.-L., and Chen Tzong-Yueh.? 2003.? Development of Nonspecific Binding Sequence and Non-cell Specificity for DNA Delivery System. Eleventh Symposium on Recent Advances in Cellular and Molecular Biology. Kenting, Taiwan, Abs. #P52

18.Chen Y-M, Yang H.-L., and Chen Tzong-Yueh.? 2003.? cDNA clonging of Myxovirus Resistance (Mx) Gene, an INF-inducible Antiviral Protein from the Grouper (Epinephelus coioides). The 2003 Annual Conference of the Fisheries Society of Taiwan. Kaohsiung, Taiwan, Abs. #B-3-1

19.Huang S-I, Lin S-C, Yang H.-L., and Chen Tzong-Yueh.? 2004.? Development of a Universal DNA Delivery System. Twelfth Symposium on Recent Advances in Cellular and Molecular Biology. Kenting, Taiwan, Abs. #P106

20.Chuang H-C, Chen Y-M, Yang H.-L., and Chen Tzong-Yueh.? 2004.? Study the Antimicrobial activity of Penaeidins. The 19th Joint Annual Conference of Biomedical Sciences, Taipei, Taiwan, Abs. #P16.

21.Chen Y-M, Mao M-H, Su Y-L, Chuang H-C, Yang H.-L., Lin H-Y, and Chen Tzong-Yueh.? 2004.? Proteomic Approach Identifies Small Heat Shock Protein as a Potentially Biomarker to Monitor Nervous Necrosis Virus Infection. 2004 International Conference of Advanced Biotechnology-Dialogue between Proteomics and Biotechnology, Tainan, Taiwan, Abs. #P2.

22.Chen Y-M, Su Y-L, Yang H.-L., and Chen Tzong-Yueh.? 2004.? The Characterization and Expression of the Mx Protein in the Grouper Cell Line. The 2004 Annual Conference of the Fisheries Society of Taiwan, Taipei, Taiwan, Abs. #A-1-2.

23.Chen Y-M, Wei C-Y, Chang H-W, Yang H.-L., and Chen Tzong-Yueh.? 2004.? Cloning, Characterization and Application of the Grouper (Epinephelus coioides) Myostatin Gene. The 2004 Annual Conference of the Fisheries Society of Taiwan, Taipei, Taiwan, Abs. #A-5-6.

24.Chen Y-M, Mao M-H, Su Y-L, Chuang H-C, Yang H.-L., Lin H-Y, and Chen Tzong-Yueh.? 2004.? Proteomic Approach Identifies Small Heat Shock Protein as a Potentially Biomarker to Monitor Nervous Necrosis Virus Infection. The 2004 Annual Conference of the Fisheries Society of Taiwan, Taipei, Taiwan, Abs. #E-C5.

25.Lin H-Y, Chen Tzong-Yueh, Chen M-S, Chen H-E, Su Y-L, ChouT-I, Su M-S, and Yang H.-L. 2004. Development of a Multivalent Bacterial Vaccine for Vibrio alginolyticus, V. parahaemolyticus and Photobacterium damselae Subspecies piscicida and Its Evaluation in Cobia (Rachycentron canadum) . The 2004 Annual Conference of the Fisheries Society of Taiwan, Taipei, Taiwan, Abs. #E-32.

26. Keynote speaker: Development of disease prevention system for warm water fish aquaculture, 2004. 2004 International Conference of Marine science and technology, 13-14, 2004, Kaohsiung, Taiwan

27. Invited speaker: The development of an oral Nervous Necrosis virus vaccine and its efficacy test in grouper, International Biotech Conference & Exhibition (IBC&E) Nov. 25-27, 2004, Kaohsiung, Taiwan

28. Huang S-I, Yang  H.-L., and Chen Tzong-Yueh.  2005. Development of a Universal Biological Materials Delivery System. The 20th Joint Annual Conference of Biomedical Sciences, Taipei, Taiwan, Abs. #P38

29. Su Y-L, Chen Y-M, Yang H.-L., and Chen Tzong-Yueh.  2005.  The Characterization and Expression of the Mx Protein in the Grouper Cell Line. Thirteenth Symposium on Recent Advances in Cellular and Molecular Biology. Kenting, Taiwan, Abs. #P111.

30. Chen Y-M, Wei C-Y, Chang H-W, Yang H.-L., and Chen Tzong-Yueh.  2005.  Cloning of the Grouper (Epinephelus coioides) Myostatin Gene. The 2005 Annual Conference of the Fisheries Society of Taiwan, Taipei, Taiwan, Abs. #BO-12.

31. Chen Y-M, Su Y-L, Shie P-S, Yang H.-L., and Chen Tzong-Yueh.  2005.  Resistance to Nodavirus Activity by Coat Protein Interaction with the Interferon-regulated GTP-binding Proteins, Grouper Mx. The 2005 Annual Conference of the Fisheries Society of Taiwan, Taipei, Taiwan, Abs. # BO-13.

32. Ou M-C, Chuang H-C, Chen Y-M, Lee G-B, Lee S-S, Yang H.-L., and Chen Tzong-Yueh.  2005.  Development of Diagnostic methods for Nervous Necrosis Virus. The 2005 Annual Conference of the Fisheries Society of Taiwan, Taipei, Taiwan, Abs. # AP-6.

33. Chien C-H, Chen Y-M, Wei C-Y, Yang H.-L., and Chen Tzong-Yueh.  2005.  Functional Analysis of the Grouper Myostatin Gene Promoter and the Application of Myostatin in Grouper. The 2005 Annual Conference of the Fisheries Society of Taiwan, Taipei, Taiwan, Abs. #BP-7.

34. Shie P-S, Chen Y-M, Su Y-L, Yang H.-L., and Chen Tzong-Yueh.  2005.  The Characterization and Expression of the Mx Protein in the Grouper Cell Line. The 2005 Annual Conference of the Fisheries Society of Taiwan, Taipei, Taiwan, Abs. # BP-15.

35. Chen Y-M, Su Y-L, Shie P-S, Yang H.-L., and Chen Tzong-Yueh.  2006.  Modulation of Nodavirus Coat Protein Localization by Binding to the Interferon-regulated GTP-binding Proteins, Grouper Mx. Fourteenth Symposium on Recent Advances in Cellular and Molecular Biology. Kenting, Taiwan, Abs. #P101.

36. Liu Y-R, Huang S-I, Yang H.-L., and Chen Tzong-Yueh.  2006.  Characterization and Functional Analysis of a Delivery System Mediated by Protein Transduction Domain. The 21th Joint Annual Conference of Biomedical Sciences, Taipei, Taiwan, Abs. #P649.

37. Chen Tzong-Yueh, Chen Y-M, and Yang H.-L.. 2006. Identification and Characterization of the Responsive Genes in Grouper for Nervous Necrosis Virus Infection. 2006 Symposium on Biotechnology Development in Taiwan: Biotechnology in Aquaculture, Tainan, Taiwan, Abs. #S10.

38. Chen Y-M, Su Y-L, Shie P-S, Yang H.-L., and Chen Tzong-Yueh. Inhibition of Nodavirus Activity by Binding Directly to the Mx Protein in Grouper (Epinephelus coioides) Cell. Aquaculture 2006, Florence, Italy, Abs

39.Ou M-C, Chuang H-C, Chen Y-M, Lee G-B, Lee S-S, Yang H.-L., and Chen Tzong-Yueh. 2006. Development of Fast and Sensitive Diagnostic Methods for Nneverous Necrosis Virus by Using Microfluidic Chip and Real-time PCR. Aquaculture 2006, Florence, Italy, Abs.

40. Chuang H-C, Chen Y-M, Lin H-Y, Yang, H.-L., and Chen T-Y*. 2006. Detection of Nerve Necrosis Virus by a Real-time PCR Method and Characterization of Its Tissue Distributions in Giant Grouper (Epinephelus lanceolatus). VNN 2006 First International Symposium on Viral Nervous Necrosis of Fish, Hiroshima, Japan, Abs.#PP-4.

41. Ou M-C, Chen Y-M, Jeng M-F, Yang, H.-L., and Chen T-Y*. 2006. Identification of Orange-spotted Grouper (Epinephelus coioides) Nervous Necrosis Virus (OGNNV) B2-Arg53 as a Critical Residue for dsRNA Binding Activity. VNN 2006 First International Symposium on Viral Nervous Necrosis of Fish, Hiroshima, Japan, Abs.#PP-7.

42. Chen Y-M, Su Y-L, Shie P-S, Yang, H.-L., and Chen T-Y*. 2006. Inhibition of Nodavirus Activity by Binding Directly to the Interferon-regulated GTP-binding Proteins, Grouper Mx. The 40th Annual Meeting of the Taiwan Society of Microbiology, Taipei, Taiwan, Abs. #V-4.

43. Chen Y-M, Su Y-L, Shie P-S, Yang, H.-L., and Chen T-Y*.  2006.  Resistance to Nodavirus Activity by Coat Protein Interaction with the Interferon-regulated GTP-binding Proteins, Grouper Mx. The 2006 Annual Conference of the Fisheries Society of Taiwan, Keelung, Taiwan, Abs. # BO-16.

44. Liu Y-R, Ou M-C, Chen Y-M, Yang, H.-L., and Chen T-Y*.  2006.  Relations Between Structure and RNAi Inhibition Activity of Nervous Necrosis Virus B2 Protein. The 2006 Annual Conference of the Fisheries Society of Taiwan, Keelung, Taiwan, Abs. # APN-1.

45. Chen Y-M, Su Y-L, Shie P-S, Yang, H.-L., and Chen T-Y*.  2006.  Resistance to Nodavirus Activity by Coat Protein Interaction with the Interferon-regulated GTP-binding Proteins, Grouper Mx. The 2006 Annual Conference of the Fisheries Society of Taiwan, Keelung, Taiwan, Abs. # BP-6.

46. Tasi T-J, Ou M-C, Chen Y-M, Lee G-B, Lee S-S, Yang, H.-L., and Chen T-Y*.  2006.  Fast Detection Method Using NNV RNA1 as Target by Microfluidic Chip. The 2006 Annual Conference of the Fisheries Society of Taiwan, Keelung, Taiwan, Abs. # BPN-14.

47. Yu-Chin Su, Yang, H.-L., Jiann-Ruey Hong*. 2006. RGNNV B2 as a novel death gene induces grouper liver cell death. 2006 The 21th Joint Annual Conference of Biomedical Sciences March 18-19; O058, p. 74.

48. Chien-Li Chiu, Jen-Leih Wu, Yang, H.-L. and Jiann-Ruey Hong*. 2006. IPNV VP3 as a novel viral death gene, targeting to ER and induces apoptosis through caspase activation in a zebrafish cell line. 2006 The 21th Joint Annual Conference of Biomedical Sciences, March 18-19; O063, p. 76.

49. Po-Chun Chen, Jen-Leih Wu, Yang, H.-L. and Jiann-Ruey Hong*. 2006.  Src tyrosine kinase may mediate in IPNV-induced apoptosis cell death. 2006 The 21th Joint Annual Conference of Biomedical Sciences, March 18-19; P674, p. 276.

50. Speaker：S. P. Chen, Yang, H.-L., J. L. Wu and J. R. Hong. 2006. NOV 28-DEC 1. Betanodavirus induces mitochondria-mediated cell death in grouper cells.VNN 2006-First international symposium on viral nervous of fish. PP6. Hiroshima, Japan.

51. Y. C. Su, Yang, H.-L.,  J. L. Wu and J. R. Hong. 2006.  Cloning and characterization of a non-structural protein B2 as a death inducer from betanodavirus. VNN 2006-First international symposium on viral nervous of fish. PP6. Hiroshima, Japan.

52. Yang, H.-L., H.Y. Lin and C.C. Lin. 2006. Nov 28. The development of an oral NNV vaccine and its efficacy evaluation in the larvae of Epinephelus coioides. 1st International symposium on viral nervous necrosis of fish, Hiroshima, Japan.

53. H.Y Lin, Y.M Chen, M.S. Chen, C.C. Lin and Yang, H.-L.. 2006. Nov 28. Mornitoring nervous necrosis virus infection in a commercil grouper hatchery in Taiwan. 1st International symposium on viral nervous necrosis of fish, Hiroshima, Japan.

54. C.C. Lin, M.S. Chen, H.Y. Lin and Yang, H.-L.. 2006. Nov 28. Establishment of experimental challenge model of grouper (Epinephelus coioides) for NNV. 1st International symposium on viral nervous necrosis of fish, Hiroshima, Japan.

55. Chen Y-M, Su Y-L, Shie P-S, Yang, H.-L., and Chen T-Y*.  2007.  The Role of Grouper Mx Confers Resistance to Nodavirus. Fifteenth Symposium on Recent Advances in Cellular and Molecular Biology. Kenting, Taiwan, Abs. #P107.

56. Yang, H.-L., Chen T-Y*, and Liu Y-R.  2007.  Production and Characterization of a Peptide-Based DNA Delivery System. The 22th Joint Annual Conference of Biomedical Sciences, Taipei, Taiwan, Abs. #P539.

57. Chen Y-M, Yang, H.-L., and Chen T-Y*.  2007. Identification of Responsive Proteins for Nervous Necrosis Virus Infection by Proteomic Approach. The Seventh International Symposium on Fish Immunology. Stirling, Scotland, Abs. #P38.

58. Invite speaker: The development of an oral NNV vaccine and its application in commercial hatchery, The 3rd Malaysia international Agro-Bio Business Conference, 12-13 , 2007 Kuala Lumpur, Malaysia

59. Session leader: The agriculture and aquaculture biotechnology and opportunity, The 3rd Malaysia international Agro-Bio Business Conference, 12-13 , 2007 Kuala Lumpur, Malaysia

60. Invited speaker: The control of NNV infection by an oral vaccine, Asian-Pacific Aquaculture 2007 Conference, Hanoi, Aug.6-9, 2007

61.Invite speaker: The development of an oral NNV vaccine and its application in commercial hatchery, The 3rd Malaysia international Agro-Bio Business Conference, 12-13, 2007 Kuala Lumpur, Malaysia

62. Session leader: The agriculture and aquaculture biotechnology and opportunity, The 3rd Malaysia international Agro-Bio Business Conference, 12-13 , 2007 Kuala Lumpur,   Malaysia

63. Invited speaker: The control of NNV infection by an oral vaccine, Asian-Pacific Aquaculture 2007 Conference, Hanoi, Aug.6-9, 2007

64. Chen Y-M, Yang, H.-L., and Chen T-Y*. 2007. Identification of Responsive Proteins for Nervous Necrosis Virus Infection by Proteomic Approach. The Seventh International Symposium on Fish Immunology. Stirling, Scotland, Abs. #P38.

65.Lin H.-Y., Chen Y.-M. , Wu P.-F., Chen J.-Y., and Yang H.-L. 2008. Development a gene-deleted subunit vaccine of Aeromonas hydrophila. WAS 2008, Busan, Korea.

b.國內會議

1.      Invited speaker 。2001。 水產動物疾病防治生物技術，臺灣水產學會年會Nov. 24, 2001

2.      許維儀，王俊傑，林翰佑，陳宗嶽，楊惠郎，以海鱺rag-1及 Ig基因之表現探討其免疫機制之發育, 九十一年度水產學會年會

3.      許維儀，王俊傑，林翰佑，陳宗嶽，楊惠郎，海鱺重要免疫基因rag-1及 Ig部份基因之選殖及序列比對, 九十一年度水產學會年會

4.                       周宜黎、吳金洌、楊惠郎、洪健睿。2004 。傳染性胰臟壞死病毒凋亡基因VP3藉由活化caspase-8  和-3 使細胞凋亡。中華民國微生物學會九十三年度學術論文發表會摘要。第16頁。

5. 洪健睿、官柏志、楊惠郎、吳金洌。2004 。胰臟壞死病毒感染能活化轉錄因子NF-kB去促使細胞凋亡和降低病毒蛋白表現之双功能角色。臺灣水產學會九十三年度學術論文發表會摘要。第3頁。

6. 蘇郁清、楊惠郎、吳金洌、洪健睿。2004 。神經壞死病毒非結構性蛋白基因B2可誘發石斑魚肝臟細胞株凋亡及可被小片段干擾性RNA減緩其功能。臺灣水產學會九十三年度學術論文發表會摘要。第10頁。

7. 曹鳳儒，林翰佑，楊惠郎. 2006. Dec 16.  點帶石斑魚幾丁質脢純化，功能性分析及基因選殖. 台灣水產年會學術論文發表會. 基隆，台灣。

8. 吳佩芬，林翰佑，楊惠郎. 2006. Dec 16.  Aeromonas hydrophia 的Aerolysin毒素之毒性分析. 台灣水產年會學術論文發表會. 基隆，台灣。

9.陳俐伽、蘇郁清、楊惠郎、吳金洌、洪健睿。2006. 證實神經壞死病毒B1為新表現之基因及其細胞內分佈之研究。2006台灣水產學會。BO-25; pp24。

10.Chen Y-M, Su Y-L, Shie P-S, Yang, H.-L., and Chen T-Y*.  2007.  The Role of Grouper Mx Confers Resistance to Nodavirus. Fifteenth Symposium on Recent Advances in Cellular and Molecular Biology. Kenting, Taiwan, Abs. #P107.

11. Yang, H.-L., Chen T-Y*, and Liu Y-R.  2007.  Production and Characterization of a Peptide-Based DNA Delivery System. The 22th Joint Annual Conference of Biomedical Sciences, Taipei, Taiwan, Abs. #P539.

12. Chen Y-M, Shie H-P, Yang H-L, and Chen T-Y*. 2007. Identification of Responsive Proteins for Nervous Necrosis Virus Infection by Proteomic Approach. Taiwan Proteomics Society International Conference 2007. Tainan, Taiwan, Abs. #P83.

13. Chen Y-M, Shie H-P, Yang H-L, and Chen T-Y*.  2007.  Proteome analysis of viral nervous necrosis grouper by two-dimensional electrophoresis. The 2007 Annual Conference of the Fisheries Society of Taiwan, Kaohsiung, Taiwan, Abs. # BO-2-2.

14. Chen Y-M, Shie P-S, Yang H-L, and Chen T-Y*.  2008. Roles of grouper Mx in regulation of cell cycle and resistance to nodavirus. The 7th Symposium on Diseases in Asian Aquaculture. Taipei, Taiwan, Abs. CP#35.

15. Chen Y-M, Shie H-P, Yang H-L, and Chen T-Y*.  2008.  Identification of small heat shock protein as stress-induced molecular chaperone-like activity in nodavirus infected grouper. The 7th Symposium on Diseases in Asian Aquaculture. Taipei, Taiwan, Abs. PB#159.

16. Tasi T-J, Lee S-S, Chen Y-M, Lee G-B, Yang H-L, and Chen T-Y*.  2008.  One step multiplex diagnosis for grouper pathogens detection. The 7th Symposium on Diseases in Asian Aquaculture. Taipei, Taiwan, Abs. PB#98.

17. Chen L-J, Su Y-C, Yang, H-L. Wu J-L and Hong J-R . 2007. RGNNV B1 protein plays an anti-apoptotic function with RGNNV infection in grouper fish cells. 2007 The 22th Joint Annual Conference of Biomedical Sciences, Taiwan. P607. [優秀壁報論文獎]

18. Chen L-J, Su Y-C, Yang, H-L. Wu J-L and Hong J-R . 2007. Identification of RGNNV protein B1 as a novel anti-apoptotic death gene. Annual meeting of the fisheries society of Taiwan, AO-2-3.

19. Shih C.-H., Liu H.-C., Wu C.-L., Lin C.-C., Chen Y.-M., Lin H.-Y., and Yang H.-L. 2008. Vibrio expression system: a novel host for nervous necrosis virus subunit vaccine production. DAA VII, Taipei.

20. Chang C-W, Chen L-J, Su Y-C, Yang, H-L. Wu J-L and Hong J-R. 2008. Identification of a novel antiapoptotic protein B1 encoded by the RGNNV TN1 strain in fish cells. The 7th Symposium on Diseases in Asian Aquaculture, June 22-26, Taipei, Taiwan. PB60

21. 施慶宏，劉曉謙，吳政隆，林青丘，陳逸民，林翰佑，楊惠郎。2007。利用弧菌表現神經壞死病毒口服次單位疫苗之有效性評估。台灣水產學會96年度論文發表會，論文摘要。

C. 專書及專書論文

1. 動物疫苗：疫苗發展技術與實驗，教育部民國九十三年。
2. 水產用疫苗：疫苗發展技術與實驗，教育部民國九十二年。
3. 我國生技產業發展策略之分析及建議, (科技發展政策報導月刊中華民國九十年十月SR 9010 PP. 761-765) 。
4. 魚用疫苗, (生物技術與產業發展，九州出版社) 。
5. 戴  謙，楊惠郎。(2001)。我國生技產業發展策略之分析及建議。科技發展政策報導。SR90010，P761-765。
6. 楊惠郎、林青丘、林翰佑。石斑稚魚NNV口服疫苗之研發：生物科技產學論壇2005秋季號 PP.17-22。國立中興大學生物科技發展中心。
7. 楊惠郎、林青丘、林翰佑。疫苗於水產養殖產業之重要性：生物科技產學論壇2005秋季號，2005年9月。 PP.24-30。國立中興大學生物科技發展中心。
8. 楊惠郎。海洋養殖50年後可能取代海上捕撈：海洋高雄期刊第5期，2005年6月。PP.39-42。高雄市政府海洋局。
9. 楊惠郎。魚用疫苗及疾病預防系統之建立：海洋高雄期刊第6期，2005年9月。PP.39-41。高雄市政府海洋局。
10. 楊惠郎。從鮭魚經驗看台灣海鱺的國際潛力：農業生技產業季刊2005年第2期。PP.23-24。財團法人生物技術開發中心。
11. 楊惠郎.2006.生生不息  應用微生物學與生物技術之傳承.九州圖書公司.105-117
12. 楊惠郎, 2007. 可在稚魚促製免疫保護力之口服疫苗. 成大榕園 第一卷第七期 - 2007年10月5日
13. 楊惠郎. 2007. 推動生技產業之我見. 成大榕園 第一卷 第四期 - 2007年9月 14日
14. 96年度科技年鑑：生物技術領域
15. 楊惠郎.2007.「多檢體快速結合並核酸探針檢驗法」技轉亞洲基因科技股份有限公司，技轉金額100萬

研究計畫 (至少五年內)

A. 國科會計畫

1. 石斑魚優質種苗之功能性基因研究--石斑魚與細菌交互作用之功能性基因體研究(2003-2004)
2. 疫苗計畫--嚴重急性呼吸道症候群病毒具中和抗體產生能力疫苗抗原及疫苗研究(2004)
3. 魚免疫系統發育之研究(第三年) 國科會(2003-2004)
4. 魚免疫系統發育之研究(第二年) 國科會(2002-2003)
5. 魚免疫系統發育之研究(第一年) 國科會(2001-2002)
6. 開發石斑魚虹彩病毒疫苗 國科會(2002-20003)
7. 建立南部生技產業聚落及辦法規劃案 國科會(2001-2002)
   

C. 一般建教案

1. 海鱺Streptococcus診斷試劑的開發 (農委會,2004)
2. 蝦口服促進劑傳遞技術之開發 (產學合作富立洋,2004)
3. 以免疫方法生產無腦神經壞死病病毒之石斑魚魚苗 (產學合作富立洋,2004)
4. 蝦口服促進劑傳遞技術之開發 (產學合作富立洋,2003)
5. 應用生物技術研發魚類感染應胰臟潰傷性疾病次單位疫苗 (產學合作富立洋,2003)
6. 冬蟲夏草保健食品之研發 - 冬蟲夏草菌株DNA圖譜分子檢定方法之建立及其運用(台灣糖業公司,2002-2003)
7. 海鱺細菌性疫苗之田間試驗(農委會,2002)
8. 生物技術科技教育改進計畫-動物基因轉殖與疫苗發展技術教學資源中心(教育部,2002)
9. 海鱺免疫檢驗試劑之研發 (農委會,2001)
10. 推動國立大學研究所基礎教育重點改進計畫-成功大學生物科技研究暨教育改進計畫(教育部,2001)
11. 水產種苗認證體系之建立-Ⅰ(水產種苗協會,2001)
12. 魚DNA疫苗關鍵技術之開發與應用(生物技術國家型計畫,1999-2002)
13. 水產養殖疫苗策略規劃(工業局,1999-2000)
14. 應用生物技術研發魚類感染性胰臟潰瘍性次單元疫苗(生物技術國家型計畫,1998-2001)

指導學生情況 (至少五年內)

A. 指導學生

1. 陳永茂(博三)、何立平(博二)、尤建智(博士)(休學中)
2. 碩一: 曹鳳儒
3. 碩二: 吳佩芬、劉曉謙、呂春美、孟倖妃
4. 碩士92級: 吳政隆
5. 碩士91級: 林青丘、康郁婷
6. 碩士90級: 許維儀、林素卿
7. 碩士89級:王俊傑、陳惠恩

B. 指導學生之特殊榮譽

1. 指導學生榮獲2002年Hewlett Parker & Far Eastern Economic Review合辦之“ Young Inventor Award: Bronze Award”(我國首次得獎)

主辦及參與國際會議 (至少五年內)

1. 主持人,臺灣生物科技產學新知研討會,台灣台南, Apr. 1-2, 2002
2. 主持人,中草藥科技化研討會, Dec. 9, 2000
3. 主持人,中草藥產業研討會, Apr. 2, 1999
4. 中日水產養殖科技研討會,日本,
5. 第九屆歐洲魚病專家協會國際會議,希臘, Sep. 17, 1999
6. 台日水產生物科技研討會, 日本, Mar. 11-17, 2000
7. 第十屆歐洲魚病專家協會國際會議, 愛爾蘭, Sep. 10-14, 2001
8. 第六屆亞洲水產學會年會, 台灣高雄, Nov. 25-30
9. 國際農業生物科技研討會,台灣台南, Dec. 13-14